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KMID : 0357119930150010025
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Korean Journal of Immunology
1993 Volume.15 No. 1 p.25 ~ p.35
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Brain Innate Immunity -Phagocytic Function, Reactive Oxygen and Nitrogen Intermediates Production from Murine Brain Microglical Cells-
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Jun Chang-Duk
Kim Tae-Young
Lee Bok-Soo
Kim Jong-Moon
Lee Won
Chung Hun-Taeg
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Abstract
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Formerly the brain was considered as an immunologically privileged organ. However, recent reports showed that certain cells of the brain might be involved in the immunological processes in the brain. For exemple, some cells of the brain could present antigen to T lymphocytes, express class II major histocompatibility complex antigens on their surfaces, and secrete such cytokines as interleukin-1 and -3 molecules. In addition, some cells of the brain possess receptors for the Fc portion of immjunoglobulins.
In this study, the author tried to purify the microglial cells from the isolated glial cells of brain of new born mice, and anaalyze the functions of the microglial cells. The purified mucroglial cells showed above 95% of F4/80 or pan maacrophage marker by immunohistochemical staining. The cells could phagoytose fluorescent particles that could be measured by the use of flow cytometer and also produce such reactive oxygen intermediates as superoxide and hydrogen peroxide that could be measured by lumonometer after stimulation with phorbol myristate acetate (PMA). In addition, these cells could produce nitric oxcide(NO) that could be measured by spectrophotometer after stimulation with murine γ-intereron(γ-IFN). Although lipopolysacharide(LPS) did not induce NO synthesis on its own, incubation of microglia cells with γ-IFN n the presence of LPS augmented NO production in dose dependent manner. It coould be inhibited prevesibly or irreversibly by NG-monomethyl-L-arginine (NGMMA), N-amino-l-arginine(NAA), arginase or diphenyleneiodonium(DPI).
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KEYWORD
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Microglical cells, Reacive oxygen intermediates, Nitric oxide
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